Cichorium intybus, commonly known as chicory root, is used as a coffee substitute and grown as a crop for livestock, but little is known about the nutritional value of chicory seeds. Therefore, the chemical compositions of Puna Chicory and Commander Chicory seeds were investigated in this study. The results revealed that the two chicory seeds contained substantial amounts of crude proteins (over 19 %), crude fat (over 22 %) and carbohydrate (over 31 %), respectively. The protein contents were two times higher than those of corn grains, and the fat contents were markedly higher comparable to alfalfa seeds. Chicory seeds were rich of most essential amino acids, and the total amino acid content of Puna Chicory seeds was higher than that of Commander Chicory seeds or alfalfa seeds. The essential fatty acid, linoleic acid was the predominant fatty acid accounted for over 76 % of the total fatty acids in the two chicory seeds, with lower saturated/unsaturated ratios (about 0.11) making them potentially a superior source of nutritional oil. Compared with alfalfa, mineral analysis showed that chicory seeds possess higher K, Ca, P, Mg, Cu, Zn and Mn elements. Overall, the study suggested that the two forage-type chicory seeds had high levels of nutritionally important components and may be of significant importance in the formulation of diets for human and animals.